Biolegend, 127617, PE/Cyanine7 anti-mouse Ly-6G Antibody, 25μg

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Ly-6G transfected EL-4J cell line.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416. Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).
Application References(PubMed link indicates BioLegend citation): Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC) Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC) Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC) Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC) Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC) Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC) Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete) Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete) Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete) Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed Ma C, et al. 2012. J. Leukoc. Biol. 92:1199. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Furuya Y, et al. 2014. J Virol. 88:9166. PubMed Dudek M, et al. 2015. Mucosal Immunol. . PubMed Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed Olofsen PA, et al. 2022. Cells. 11:. PubMed Solier S, et al. 2023. Nature. 617:386. PubMed Vereertbrugghen A, et al. 2023. J Neuroinflammation. 20:120. PubMed Wang Z, et al. 2023. Mediators Inflamm. 2023:9810733. PubMed Zhao C, et al. 2023. NPJ Regen Med. 8:29. PubMed Huang T, et al. 2023. Cell Death Dis. 14:354. PubMed Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed Bernasconi R, et al. 2021. EMBO Mol Med. :e14392. PubMed Buckland RL, et al. 2020. Curr Protoc Mouse Biol. 10:e78. PubMed Zhang Y, et al. 2022. J Cereb Blood Flow Metab. 42:952. PubMed Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Martin-Martin I, et al. 2022. Cell Rep. 39:110648. PubMed Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed Miró L, et al. 2022. Cancers (Basel). 14: . PubMed Ullah I, et al. 2023. Cell Rep Med. 4:100893. PubMed Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed Fan R, et al. 2023. J Immunother Cancer. 11: . PubMed Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed Li XF, et al. 2023. J Exp Med. 220: . PubMed Kang J, et al. 2023. JCI Insight. 8: . PubMed Schick J, et al. 2023. Elife. 12: . PubMed Hu R, et al. 2023. Cell Death Discov. 9:50. PubMed Israelow B, et al. 2020. bioRxiv. . PubMed Bieren J, et al. 2015. J Immunol. 194:1154. PubMed Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed Buxadé M, et al. 2018. J Exp Med. 215:2901. PubMed Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed Lin R, et al. 2020. Sci Rep. 10:14397. PubMed Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed Douglas T, et al. 2015. J Immunol. 195: 2365-2373. PubMed Mercer HL, et al. 2020. PLoS Pathog. 16:e1008572. PubMed Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed Zhao J, et al. 2019. Nat Commun. 10:899. PubMed Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed Sanfilippo A, et al. 2015. Infect Immun . 83: 2976-2983. PubMed Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed Jeong D, et al. 2021. eLife. 0.416666666666667. PubMed Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed Boothby IC, et al. 2021. Nature. 599:667. PubMed Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed Lu S, et al. 2020. JCI Insight. 5:00. PubMed Philip N, et al. 2016. PLoS Pathog. 12:e1005910. PubMed Park Y, et al. 2013. Cancer Res . 73:5669. PubMed Campana L, et al. 2018. J Immunol. 200:1169. PubMed Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed Ma YV, et al. 2021. MAbs. 13:2003281. PubMed Sitnik S, et al. 2020. Mol Ther Oncolytics. 17:190. PubMed Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed Donlan AN, et al. 2020. Anaerobe. 66:102275. PubMed Yu X, et al. 2022. J Extracell Vesicles. 11:e12185. PubMed Dane EL, et al. 2022. Nat Mater. 21:710. PubMed Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed Singh S, et al. 2021. Cell Host Microbe. 29(3):347-361.e12. PubMed Yin S, et al. 2016. Nat Commun. 7: 11311. PubMed Chan C, et al. 2012. Hypertension. 60:1207. PubMed Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed Guo L, et al. 2021. Front Immunol. 12:785457. PubMed Stump CT, et al. 2021. Open Biol. 11:210245. PubMed Galvani S, et al. 2015. Sci Signal. 8: ra79. PubMed Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed Lasso P, et al. 2020. Front Immunol. 584959:11. PubMed Sun KT, et al. 2018. Front Physiol. 1.06875. PubMed Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed Guo P, et al. 2021. J Immunol. 207:408. PubMed Nguyen GT, et al. 2021. iScience. 24(8):102871. PubMed AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed Coquery C, et al. 2014. PLoS One. 9:102284. PubMed Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed Kaufmann E et al. 2018. Cell. 172(1-2):176-190 . PubMed Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed Yang Y, et al. 2022. Elife. 11:. PubMed Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed Lei J, et al. 2021. Theranostics. 4251:11. PubMed Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed Bryant J, et al. 2014. J Immunol. 192:6092. PubMed Fischer KJ, et al. 2019. J Immunol. 202:2027. PubMed Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed Poczobutt JM, et al. 2021. Am J Respir Cell Mol Biol. 64:629. PubMed Ding P, et al. 2022. Bone Res. 10:42. PubMed Almishri W, et al. 2015. J Hepatol. 63: 943-951. PubMed Nair S, et al. 2021. JCI Insight. 6:. PubMed Bravo-Caparrós I, et al. 2020. FASEB J. 34:5951. PubMed Lingegowda H, et al. 2022. Front Reprod Health. 3:726936. PubMed Chen F, et al. 2016. J Immunol. 196: 4390 - 4399. PubMed Moore J, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 906-917. PubMed Johansson J, et al. 2013. J Neurosci. 33:16016. PubMed Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed Ngamsri KC, et al. 2020. Front Immunol. 11:407. PubMed Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed Upadhya R, et al. 2016. MBio. 7: 00547-16. PubMed Alexander K, et al. 2016. Immunol Cell Biol. 95:7-16. PubMed Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed Wong NR, et al. 2021. Immunity. 54:2072. PubMed Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed Li N, et al. 2022. Nat Commun. 13:7281. PubMed Wang X, et al. 2021. Cell. 184:5357. PubMed Xiao Y, et al. 2021. Cell. 184:6037. PubMed Casselli T, et al. 2021. PLoS Pathog. 17:e1009256. PubMed Ling Y, et al. 2016. Pharmacol Res. S1043-6618(16)31329-9. PubMed Nguyen GT, et al. 2020. eLife. 9:e56656.. PubMed Tang W, et al. 2021. Front Cell Dev Biol. 9:728057. PubMed Tang S, et al. 2022. Virol J. 19:32. PubMed Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed Braza MS et al. 2018. Immunity. 49(5):819-828 . PubMed White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed Wang X, et al. 2021. Theranostics. 11:209. PubMed Lee J, et al. 2015. Nat Commun. 6: 8499. PubMed Goc J, et al. 2021. Cell. 184:5015. PubMed Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed Dietschmann A, et al. 2020. Eur J Immunol. 50:1044. PubMed Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed Wu L, et al. 2021. Cell Death Dis. 12:1064. PubMed
RRID: AB_1877262 (BioLegend Cat. No. 127617) AB_1877261 (BioLegend Cat. No. 127618)
Structure: A 21-35 kD GPI-anchorded membrane protein
Distribution: Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
Cell Type: Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Antigen References: Fleming TJ, et al. 1993. J. Immunol. 151:2399.
Gene ID: 546644
UniProt: View information about Ly-6G on UniProt.org
Clone: 1A8
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus G
Isotype: Rat IgG2a, κ