Biolegend, 127810, APC anti-mouse TCR Vα2 Antibody, 100μg

The TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Soluble TCR from mouse CTL clone KB5-C20
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.
Application References(PubMed link indicates BioLegend citation): Pircher H, et al. 1992. Eur. J. Immunol.. 22:399. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed Rao RR, et al. 2012. Immunity. 36:374. PubMed.
Product Citations: Thomas R, et al. 2021. Immunology. 163:478. PubMed Lutes LK, et al. 2021. eLife. 10:00. PubMed Soni C, et al. 2014. J Immunol. 193:4400. PubMed Ise W, et al. 2018. Immunity. 48:702. PubMed Kim C, et al. 2019. Cell Rep. 29:2202. PubMed Schuldt N, et al. 2015. PLoS One. 10: 0145762. PubMed Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed Huang H, et al. 2021. Cell. 184(5):1245-1261.e21. PubMed Barbet G, et al. 2018. Immunity. 48:584. PubMed Starck L, et al. 2014. J Immunol. 192:206. PubMed
RRID: AB_1089251 (BioLegend Cat. No. 127809) AB_1089250 (BioLegend Cat. No. 127810)
Distribution: On a subset of T cells
Function: In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
Interaction: Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor: TCR recognizes MHC/peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Kubo RT, et al. 1989. J. Immunol.. 142:2736. 2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.
Gene ID: 21473
UniProt: View information about TCR Valpha2 on UniProt.org
Clone: B20.1
Regulatory Status: RUO
Other Names: T cell receptor Vα 2
Isotype: Rat IgG2a, λ