Biolegend, 129005, PE anti-mouse Tim-2 Antibody, 25μg

Tim-2 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-2. It is expressed on activated T cells and preferentially on differentiated Th 2 cells. Sema4A is the ligand of Tim-2. A blockade of Tim-2/Tim-2 ligand interaction results in T cell hyperproliferation and Th 2 cytokine production. Tim-2 plays a critical role in the development of atopic disease and other Th 2 -biased immune responses.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance in each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_1227792 (BioLegend Cat. No. 129006)
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 33 kD
Distribution: Developmentally regulated; highly expressed in embryonic development at the 8-cell stage and blastocyst. Expressed on activated T lymphocytes.
Ligand/Receptor: Sema4A, H-ferritin
Cell Type: T cells, Embryonic Stem Cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.2. Kumanogoh A, et al. 2002. Nature 419:629.3. Chakravarti S, et al. 2005. J. Exp. Med. 202:437
Gene ID: 171284
UniProt: View information about Tim-2 on UniProt.org
Clone: F37-2C4
Regulatory Status: RUO
Other Names: T cell immunoglobulin and mucin domain containing protein-2
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.