Biolegend, 129817, Brilliant Violet 421™ anti-mouse CD196 (CCR6) Antibody, 125microl

CCR6 is a G-protein linked chemokine receptor which binds the chemokine CCL20/MIP-3a. It is expressed on B lymphocytes and certain subsets of dendritic cells and T cells. CCR6 is reported to be involved in mucosal immune response and lymphocyte migration and homeostasis.
125microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: N-terminal peptide (aa. 2-38) of mouse CCR6 fused with GST
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Product Citations: Cibrián D, et al. 2020. J Allergy Clin Immunol. 145:199. PubMed Kedmi R, et al. 2022. Nature. 610:737. PubMed Kathania M, et al. 2016. Nat Immunol. 10.1038/ni.3488. PubMed Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed Kawabe T, et al. 2013. J Immunol. 190:5788. PubMed Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed Teng F, et al. 2021. Cell Rep. 37:110051. PubMed Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed Wanke-Jellinek L, et al. 2016. J Leukoc Biol. 99: 483 - 493. PubMed
RRID: AB_10898320 (BioLegend Cat. No. 129817) AB_11219003 (BioLegend Cat. No. 129818)
Structure: G-protein linked, seven transmembrane, domain-spanning chemokine receptor; 367 amino acids long and about 75% homology with human CCR6
Distribution: Expressed on spleen B cells, immature splenic dendritic cells in Peyer's patches, and some T cell subset
Function: Important in regulating mucosal immune response
Ligand/Receptor: CCL20/MIP-3a
Cell Type: B cells, Dendritic cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Rossi D, et al. 1997. J. Immunol. 158:1033. 2. Lukacs N, et al. 2001. J. Exp. Med. 194:551. 3. Cook DN, et al. 2000. Immunity 12:495
Gene ID: 12458
UniProt: View information about CD196 on UniProt.org
Clone: 29-2L17
Regulatory Status: RUO
Other Names: CD196, BN-1, CKR-L3, DRY6, KY411, STRL22
Isotype: Armenian Hamster IgG
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.