Biolegend, 129908, Alexa Fluor® 647 anti-mouse Tim-4 Antibody, 100μg

TIM4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells.TIM4 binds to TIM1 to promote T cell proliferation by enhancing cell division and reducing apoptosis.TIM4 bind to phosphatidylserine through its FG-CC’ binding cleft in the N-terminal IgV domain to facilitate the clearance of apoptotic cells or bodies.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse TIM4-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706
RRID: AB_1227797 (BioLegend Cat. No. 129907)
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain
Distribution: Unlike the other TIMs, TIM4 is only expressed by antigen-presenting cells, but not by T cells.
Function: TIM4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.
Interaction: Phosphatiylserine is the ligand of TIM4 and TIM4 is a ligand of TIM1
Ligand/Receptor: Phosphatidylserine, TIM1
Cell Type: Antigen-presenting cells
Biology Area: Immunology
Antigen References: 1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577 2. Miyanishi M et al. 2007. Nature 450:435 3. Rodriguez-Manzanet R et al. 2008. J. Immunol. 180:4706
Gene ID: 276891
UniProt: View information about Tim-4 on UniProt.org
Clone: F31-5G3
Regulatory Status: RUO
Other Names: T cell immunoglobulin and mucin domain containing protein-4
Isotype: Rat IgG1, κ