Biolegend, 132206, PE anti-mouse CD155 (PVR) Antibody, 100μg

Mouse CD155, also known as homolog of polivirus receptor, is the founding member of a subfamily of immunoglobulin-like adhesion receptors (nectins). Mouse CD155 is expressed on a wide variety of cells of hematopoietic origin apart from its existence on the epithelial cells. The engagement of CD155 with CD226 and CD96 induces cytotoxicity of NK cells and CTL.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: EL4 transfected with mouse CD155
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Tahara-Hanaoka S, et al. 2004. Int Immunol 16:533 Maier MK, et al. 2007. Eur J. Immunol. 37:2214 Bottino C, et al. 2003. J. Exp. Med. 198:557 Chan CJ, et al. 2010. J. Immunol. 184;902. PubMed
Product Citations: Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed Shapiro MR, et al. 2020. Front Immunol. 1.972222222. PubMed Chan C, et al. 2010. J Immunol. 184:902. PubMed Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
RRID: AB_1279105 (BioLegend Cat. No. 132205) AB_1279103 (BioLegend Cat. No. 132206)
Structure: 70 kd immunoglobulin-like molecule with three Ig-like domains.
Distribution: CD155 is expressed at cell junctions on the primary vascular endothelial cells. Moreover, mouse CD155 is highly expressed on DP thymocytes and expression level is dropped in peripheral single positive CD4+ and CD8+ T cells. CD155 can be detected on T reg, other activated T cells and on NKT cells. Resident monocytes express moderate amount of CD155, but upregulated its expression during inflammation. Other cells, such as IEL, neutrophils and subsets of B cells were found to express CD155 to varying extents.
Function: Apart from its function of adhering junction among contacting epithelial cells, the interaction of DNAM-1 (CD226) with its ligands CD155 and CD112 (nectin 2) induces cytotoxcity of NK cells and CD8+ T cells and cytokine secretion.
Ligand/Receptor: CD226 (DNAM-1), CD96
Cell Type: B cells, Monocytes, Neutrophils, T cells, Thymocytes, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Mendelsohn C et al. 1989. Cell 56:855
Gene ID: 52118
UniProt: View information about CD155 on UniProt.org
Clone: 4.24.1
Regulatory Status: RUO
Other Names: PVR (poliovirus receptor) homolog
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.