Biolegend, 132307, APC anti-mouse CD132 (common γ chain) Antibody, 25μg

CD132 is a 64-70 kD type I transmembrane glycoprotein of the Ig superfamily, and is also known as common γ chain (γc) or IL-2 receptor γ subunit. It is expressed broadly on T- and B-lymphocytes, NK cells, monocytes, and granulocytes. CD132 is an essential component of cytokine receptors for IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21. Ligand binding induces tyrosine phosphorylation and initiates signaling through a JAK/STAT pathway. CD132 mutation results in X-linked severe combined immune deficiency (XSCID).
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Kondo M, et al. 1993. Science 262:1874 (Block)Kondo M, et al. 1994. Science 263:1453 Kimura Y, et al. 1995. Int Immunol. 7:115 (Block)Veerman KM, et al. 2012. J. Immunol. 188:1638. PubMed. Hennion-Tscheltzoff O, et al. 2013. Blood. 121:4684. PubMed
Product Citations: Zhou D, et al. 2022. Nat Commun. 13:5174. PubMed Glassman CR, et al. 2021. eLife. 10:00. PubMed Yue X, et al. 2021. EMBO Rep. 22:e52716. PubMed Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
RRID: AB_10643575 (BioLegend Cat. No. 132307) AB_10643576 (BioLegend Cat. No. 132308)
Structure: Type I transmembrane glycoprotein, Ig superfamily 64-70 kD
Distribution: T cells, B cells, NK, monocytes, granulocytes
Function: Signaling
Ligand/Receptor: Component of cytokine receptors for IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21
Cell Type: T cells, B cells, NK cells, Monocytes, Granulocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Itano M, et al. 1996. Tohoku J. Exp. Med. 178:3892. Zola H, et al. eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication 3. Nakarai T, et al. 1994. J. Exp. Med. 180:241 4. Kawahara A, et al. 1995. Proc. Natl. Acad. Sci. USA. 92:8724 5. Habib T, et al. 2002. Biochemistry. 41:8725 6. Matthews DJ, et al. 1995. Blood 85:38
Gene ID: 16186
UniProt: View information about CD132 on UniProt.org
Clone: TUGm2
Regulatory Status: RUO
Other Names: Common γ chain, γc, IL-2 receptor γ subunit
Isotype: Rat IgG2b, κ