Biolegend, 132402, Purified anti-mouse CD62E (E-selectin) Antibody, 100μg

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I transmembrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells. CD62E is involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Sialyl Lewis X (CD15s) and PSGL-1 (CD162).
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Transfectant cells (29-564 aa) and recombinant protein (29-564 aa)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Flow cytometry testing showed that clone W20055M does not affect binding of clone W20055P on target cells.
RRID: AB_3097461 (BioLegend Cat. No. 132402)
Structure: Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Distribution: Highly expressed on activated endothelial cells
Function: Involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
Ligand/Receptor: Sialyl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported to interact with CD62E
Cell Type: Endothelial cells
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Gene ID: 20339
UniProt: View information about CD62E on UniProt.org
Clone: W20055M
Regulatory Status: RUO
Other Names: E-selectin, ELAM-1, LECAM-1
Isotype: Rat IgG2b, κ