Biolegend, 133203, PE anti-mouse RAE-1δ Antibody, 50μg

RAE-1δ is one of the five RAE-1 family, GPI-linked membrane protein consisting of alpha, beta, gamma, delta, and epsilon. They are strong homology within the family, related by 92%-95% sequence identity. They are distantly related to MHC class I proteins. RAE-1 proteins are abundantly expressed in fetal tissues, but not in normal adult tissue. They are constitutively expressed on some tumors and can be induced by retinoid acid. Its ligand NKG2D is found on NK cells, many transformed cell lines, and LPS-stimulated peritoneal macrophages. The interaction of NKG2D with RAE-1 transmits stimulatory signals to NK cells and other hematopoietic cells, leading to enhanced proliferation, cytokine secretion and target killing. They are involved in the regulation of innate and immune cytotoxic responses to tumors, pathogen-infected cells, and autoimmune diseases.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse RAE-1δ transfected RMA-S cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.015 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Lodoen M, et al. 2003. J. Exp. Med. 197:1245Carayannopoulos LN, et al. 2002. Eur J. Immunol.32 (3):597
RRID: AB_1595625 (BioLegend Cat. No. 133203)
Structure: RAE is a family of GPI-linked membrane protein consisting of alpha, beta, gamma, delta, and epsilon. They are strong homology within the family, related by 92%-95% sequence identity. They are distantly related to MHC class I proteins.
Distribution: RAE-1 proteins are abundantly expressed in fetal tissues, but not in normal adult tissue. They are constitutively expressed on some tumors and can be induced by retinoid acid.
Ligand/Receptor: NKG2D
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Cerwenka A, et al. 2000. Immunity 12:7212. Lodoen M, et al. 2003. J. Exp. Med. 197:12453. Diefenbach A, et al. 2001. Nature 413:165
Gene ID: 66679
UniProt: View information about RAE-1 delta on UniProt.org
Clone: Charlotte d1.23
Regulatory Status: RUO
Other Names: Retinoid acid early inducible gene-1δ, RAE-1δ, RAE-1δ
Isotype: Mouse IgG1
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.