Biolegend, 133628, Biotin anti-mouse CD226 (DNAM-1) Antibody, 100μg

Mouse CD226 (DNAM-1) is a 65 kd glycoprotein of a member of Ig super-family adhesion molecule containing 2 immunoglobulin-like domains. It is associated with leukocyte function associated antigen-1 (LFA-1) involving in TCR-mediated signal transduction for T cell proliferation and differentiation. CD226 is also involved in NK cell and T cell mediated cytotoxicity against certain tumors. CD226 (DNAM-1) is constitutively expressed on naïve CD8 + and CD4 + T cells, macrophages, and NK cells. It was reported that CD226 expression is up-regulated on Th1 cells, but down-regulated on Th2 and Th0 cells. CD155 and CD112 are the ligands for CD226.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse DNAM-1 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Dardalhon V, et al. 2005. J. Immunol. 175:1558 Shirakawa J, et al. 2006. International Immunology 18(6):951-957
RRID: AB_2716225 (BioLegend Cat. No. 133628)
Structure: 65 kd glycoprotein of a member of Ig super-family containing 2 immunoglobulin-like domains.
Distribution: Expressed on majority of CD8+ and CD4+ T cells, NK cells, monocytes/macrophages and a subset of B cells. Reported to up-regulated on Th1 cells, but down-regulated in Th2 cells.
Function: Mediate cellular adhesion, involved in LFA-1 co-stimulatory signal for T cell differentiation and proliferation.
Ligand/Receptor: CD112 and CD155
Cell Type: B cells, Macrophages, Monocytes, NK cells, T cells, Th1
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1 Tahara Hanaoka S, et al. 2006. Blood. 107:1491 2 Shibuya K et al. 2003. J. Exp. Med. 198:1829.
Gene ID: 225825
UniProt: View information about CD226 on UniProt.org
Clone: TX42.1
Regulatory Status: RUO
Other Names: DNAM-1, PTA1 (Platelet and T cell activation antigen 1), TLISA1, LFA-1 associated molecule PTA-1
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.