Biolegend, 135007, FITC anti-mouse CD127 (IL-7Rα) Antibody, 50μg

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-7Ra-IgG1 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone A7R34 is able to completely block the binding of clone S18006K to IL-7Rα. It does not block SB/199.
Application References(PubMed link indicates BioLegend citation): Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. Hashi H, et al. 2001. J. Immunol. 166:3702. Taylor R, et al. 2007. J. Immunol. 178:5659. Mazzon C, et al. 2011. Blood. 118:2733. PubMed Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
Product Citations: Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed Dong J, et al. 2013. PLoS One. 8:e56378. PubMed Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed Bajaña S, et al. 2022. iScience. 25:103732. PubMed Giridhar P, et al. 2016. J Immunol. 197(11):4228-4239. PubMed Hebbandi Nanjundappa R, et al. 2017. Cell. 171:655. PubMed Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
RRID: AB_1937231 (BioLegend Cat. No. 135007) AB_1937232 (BioLegend Cat. No. 135008)
Structure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246. 3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
Gene ID: 16197
UniProt: View information about CD127 on UniProt.org
Clone: A7R34
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Rat IgG2a, κ