Biolegend, 135025, Brilliant Violet 605™ anti-mouse CD127 (IL-7Rα) Antibody, 125microl

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
125microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-7Ra-IgG1 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is 0.6 µg per million cells in 100 µl volume. For flow cytometric staining using the test size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone A7R34 is able to completely block the binding of clone S18006K to IL-7Rα. It does not block SB/199.
Application References(PubMed link indicates BioLegend citation): Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. Hashi H, et al. 2001. J. Immunol. 166:3702. Taylor R, et al. 2007. J. Immunol. 178:5659. Mazzon C, et al. 2011. Blood. 118:2733. PubMed Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
Product Citations: Guo A, et al. 2022. Nature. 607:135. PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Feng J, et al. 2022. Immunity. 55:405. PubMed Han SJ et al. 2017. Immunity. 47(6):1154-1168 . PubMed Qi Z, et al. 2022. Nat Commun. 13:182. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed Progatzky F, et al. 2021. Nature. 599:125. PubMed Ganguly S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:891. PubMed Lee W, et al. 2021. J Virol. 95:e0053021. PubMed Dionisio-Santos DA, et al. 2021. Front Neurosci. 15:758677. PubMed Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed Collins N, et al. 2020. Cell. 178(5):1088-1101.e15.. PubMed Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed Dingler FA, et al. 2020. Mol Cell. . PubMed
RRID: AB_2562114 (BioLegend Cat. No. 135025) AB_2572047 (BioLegend Cat. No. 135041)
Structure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246. 3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
Gene ID: 16197
UniProt: View information about CD127 on UniProt.org
Clone: A7R34
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Rat IgG2a, κ