Biolegend, 135308, Biotin anti-mouse CD135 Antibody, 500μg

CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Flt3 transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Sergejeva S, et al. 2004. Blood 103:1270. Auffray C, et al. 2009. J. Exp. Med. 206:595. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
Product Citations: Feng J, et al. 2022. Immunity. 55:405. PubMed Andersson S, et al. 2012. PLoS One. 7:e47668. PubMed Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed Chavez JS, et al. 2022. Cells. 11:. PubMed Fielding C, et al. 2022. Nat Commun. 13:543. PubMed Young K, et al. 2021. Cell Stem Cell. . PubMed
RRID: AB_1953266 (BioLegend Cat. No. 135307) AB_1953267 (BioLegend Cat. No. 135308)
Structure: A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution: Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.
Function: Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor: Flk-2/FLT3 ligand
Cell Type: B cells, Hematopoietic stem and progenitors
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Waskow C, et al.Nat. Immunol. 9:676 2. Veiby OP, et al. 1996. Blood 88(4):1256 3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953 4. Mattews W, et al. 1991. Cell. 65(7):1143 5. Hannum C, et al. 1994. Nature 368(2):643 6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478
Gene ID: 14255
UniProt: View information about CD135 on UniProt.org
Clone: A2F10
Regulatory Status: RUO
Other Names: Flk-2, Flt3, Ly-72
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.