Biolegend, 135805, PE anti-mouse CD88 (C5aR) Antibody, 50μg

CD88 is a single chain protein with seven membrane-spanning regions and a molecular weight of 43 kD. CD88 is expressed by neutrophils, eosinophils, and monocytes. There have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as G i , and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA 2 , among other molecules, resulting in chemotaxis, granule enzyme release, and superoxide anion production.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: mC5aR transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications for the relevant formats include: blocking of C5a binding.
Application References(PubMed link indicates BioLegend citation): Godau J, et al. 2004. J. Immunol. 173:3437. Shushakova N, et al. 2002. J. Clin. Invest. 110:1823. Kim SH, et al. 2013. Int Immunol. 25:623. PubMed
Product Citations: Purvis GSD, et al. 2022. Br J Pharmacol. 179:2754. PubMed Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed Mehta G, et al. 2015. J Immunol. 194:5446. PubMed Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed Monteran L, et al. 2022. Nat Commun. 13:5797. PubMed Hoogendijk AJ, et al. 2020. Cell Reports. 29(8):2505-2519. PubMed Bosmann M, et al. 2012. J Immunol. 188:5086. PubMed Kim S, et al. 2013. Int Immunol. 25:623. PubMed Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed Taylor L, et al. 2016. PLoS One. 11: 0160685. PubMed Bisht K, et al. 2014. Biochem Biophys Res Commun. 449:94. PubMed Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed Zheng QY, et al. 2019. Front Immunol. 10:1866. PubMed Giles DA, et al. 2018. J Clin Invest. 128:5322. PubMed Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed Zheng QY, et al. 2020. FASEB J. 34:10590. PubMed
RRID: AB_2067285 (BioLegend Cat. No. 135805) AB_2243735 (BioLegend Cat. No. 135806)
Structure: Single chain protein with seven membrane-spanning regions, 43 kD.
Distribution: Neutrophils, eosinophils, monocytes, glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells.
Function: After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction: CD88 is coupled to heterotrimeric G proteins such as Gi, The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
Bioactivity: Chemotaxis, degranulation.
Cell Type: Dendritic cells, Endothelial cells, Eosinophils, Monocytes, Neutrophils
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827. 2. Ippel JH, et al. 2009. J. Biol. Chem. 284:12363. 3. Monk PN, et al. 2007. Br. J. Pharmacol. 152:429. 4. Griffin RS, et al. 2007. J. Neurosci. 27:8699. 5. Lee H, et al. 2006. Nat. Biotechnol. 24:1279. 6. Buck E and Wells JA, et al. 2005. P. Natl. Acad. Sci. USA 102:2719.
Gene ID: 12273
UniProt: View information about CD88 on UniProt.org
Clone: 20/70
Regulatory Status: RUO
Other Names: C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.