Biolegend, 137021, Brilliant Violet 605™ anti-mouse CD68 Antibody, 50μg

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Purified Con A receptor glycoproteins from the P815 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested FC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.
Application References(PubMed link indicates BioLegend citation): Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB) Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC) Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC) Kayama H, et al. 2012. PNAS. 109:5010. PubMed Park S, et al. 2013. Biomaterials. 34:598. PubMed Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed Monslow J, et al. 2020. Am J Pathol. 1118:190. PubMed Reinfeld BI, et al. 2021. Nature. 593:282. PubMed Howard ZM, et al. 2021. Am J Pathol. 191:730. PubMed
RRID: AB_2616811 (BioLegend Cat. No. 137021)
Structure: A member of the lysosomal-associated membrane protein (lamp) family.
Distribution: Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.
Function: Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type: Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References: 1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833. 2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.
Gene ID: 12514
UniProt: View information about CD68 on UniProt.org
Clone: FA-11
Regulatory Status: RUO
Other Names: Macrosialin
Isotype: Rat IgG2a