Biolegend, 137229, PerCP/Cyanine5.5 anti-mouse/human Helios Antibody, 25tests

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is also involved in tumor immunity.
25tests
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Helios peptide (aa 51-107)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear™ 10X Perm Buffer (Cat. No. 424403). View more applications data for a different format of this clone in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed Verhagen J and Wraith D. 2010. J. Immunol. 185:7129. Stone B, et al. 2012. Clin Immunol. 145:153. PubMed Vaeth M, et al. 2012. PNAS. 109:16258. PubMed Angin M, et al. 2014. PLoS One. 9:86920. PubMed Bedke T, et al. 2014. Immunol Cell Biol. PubMed Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
Product Citations: Jayachandran R, et al. 2019. Immunity. 50:152. PubMed Carlsson J, et al. 2015. PLoS One. 10: 0143741. PubMed Wang L, et al. 2019. Cell Rep. 29:1848. PubMed Stathopoulou C, et al. 2020. Immunity. 49(2):247-263.e7.. PubMed Pagel J, et al. 2020. Front Immunol. 11:565257. PubMed Yang L, et al. 2020. Genes Dis. 7:128. PubMed Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
RRID: AB_2561639 (BioLegend Cat. No. 137229) AB_2561640 (BioLegend Cat. No. 137230)
Structure: A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution: Regulatory T cells (Tregs), conventional T cells, thymocytes.
Function: Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Kelly CM, et al. 1998. Curr. Biol. 8:508. 2. Dovat S, et al. 2005. J. Immunol. 175:3508. 3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167. 4. Cai Q, et al. 2009. J. Immunol. 183:2303. 5. Zhang Z, et al. 2007. Blood 109:2190. 6. Hahm K, et al. 1998. Genes Dev. 12:782.
Gene ID: 2277922807
UniProt: View information about Helios on UniProt.org
Clone: 22F6
Regulatory Status: RUO
Other Names: IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
Isotype: Armenian Hamster IgG
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.