Biolegend, 137305, PE anti-mouse/human CD351 (Fc α/µ receptor) Antibody, 50μg

Fcα/µ receptor (Fcα/µR), CD351, is a type I transmembrane protein with an Ig-like domain in extracellular portion.  It is a novel Fc receptor for IgM and IgA.  Fcα/µ receptor mRNA has been found in thymus, spleen, liver, kidney, intestines, testes, placenta, and T cell leukemia cell lines.  Fcα/µ receptor protein expression is detected on B cells, macrophages, and activated T cells.  Fcα/µ receptor mediates primary B lymphocyte endocytosis of IgM-coated Staphylococcus aureus.   It is involved in the primary stages of the immune response to microbes.
50μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: mFcα/µ receptor transfected Ba/F3 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported (for relevant formats) application include: blocking binding of both IgM and IgA to the mFca/µ receptor2.
Application References(PubMed link indicates BioLegend citation): Cho Y, et al. 2006. Biochem. Biophys. Res. Commun. 345:474. Shibuya A, et al. 2006. Springer Semin. Immunopathol. 28:377. Anguetil F, et al. 2015. J Immunol. 194:3664. PubMed
Product Citations: Biswas S, et al. 2021. Nature. 591:464. PubMed Anquetil F, et al. 2015. J Immunol. 194:3664. PubMed Legrain S, et al. 2015. J Immunol. 195: 4171 - 4175. PubMed
RRID: AB_10550805 (BioLegend Cat. No. 137306)
Structure: About 70 kD transmembrane glycoprotein with an Ig-like domain in extracellular portion.
Distribution: B cells, macrophages, and activated T cells.
Function: Mediate primary B lymphocyte endocytosis and involve in the primary stages of the immune response to microbes.
Ligand/Receptor: IgA and IgM
Cell Type: B cells, Macrophages, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Shibuya A, et al. 2000. Nat. Immunol. 1(5):441. 2. Sandor M, et al. 1992. J. Immunol.. 148(8):2363. 3. Cho Y, et al. 2010. Mol. Immunol. 47(4):878.
Gene ID: 1412583953
UniProt: View information about CD351 on UniProt.org
Clone: TX61
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Fcα/μ receptor, Fcα/μR
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.