Biolegend, 137601, Purified anti-mouse CD335 (NKp46) Antibody, 50μg

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: NKP46-IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.
Application References(PubMed link indicates BioLegend citation): Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ) Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ) Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations: Guerriero J, et al. 2011. J Immunol. 186:3517. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed Potempa M, et al. 2022. J Immunol. 208:1742. PubMed Wang C, et al. 2020. Oncoimmunology. 9:1809947. PubMed Song C, et al. 2019. Nat Commun. 10:3745. PubMed Puca E, et al. 2020. J Gastrointest Oncol. 1.015972222. PubMed Goodall KJ, et al. 2021. J Biol Chem. 297:101141. PubMed Sodir NM, et al. 2022. Nat Commun. 13:6782. PubMed Ma L, et al. 2021. Clinical Cancer Research. 27(6):1778-1791. PubMed Drzyzga A, et al. 2021. Cancers (Basel). 13: . PubMed Xiang H, et al. 2021. MAbs. 13:1981202. PubMed Cribbs AP, et al. 2021. Front Immunol. 12:626255. PubMed Kortlever RM et al. 2017. Cell. 171(6):1301-1315 . PubMed Puca E, et al. 2020. Int J Cancer. 2518:146. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Staaf E, et al. 2018. Sci Signal. 11:00. PubMed Bajic D, et al. 2020. Mol Cancer Ther. 19:2554. PubMed Goh W, et al. 2020. Cell Rep. 33:108285. PubMed Aguilar OA, et al. 2020. J Immunol. 205:1709. PubMed Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed Crinier A, et al. 2018. Immunity. 49:971. PubMed Tyagi A, et al. 2021. Nat Commun. 12:474. PubMed Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed Martin E, et al. 2020. JCI Insight. :5. PubMed Han H, et al. 2020. Cancer Cell. 36(5):483-497.e15.. PubMed Ito Y, et al. 2018. J Exp Med. 215:2617. PubMed
RRID: AB_10551441 (BioLegend Cat. No. 137601) AB_10552740 (BioLegend Cat. No. 137602)
Structure: Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution: Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
Function: NK cells activation
Ligand/Receptor: Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60. 2. Caligiuri MA. 2008. Blood 112:461. 3. Colonna M. 2009. Immunity 31:15.
Gene ID: 17086
UniProt: View information about CD335 on UniProt.org
Clone: 29A1.4
Regulatory Status: RUO
Other Names: NKp46, NCR1
Isotype: Rat IgG2a, κ