Biolegend, 138101, Purified anti-mouse CD144 (VE-cadherin) Antibody, 50μg

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo .
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: VE-cadherin protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP, WB, ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.
Application Notes: Additional reported applications (for relevant formats) include: Immunoprecipitation1, Western Blot1, functional assay2, 3 by inhibiting vascular endothelial cell-cell interactions and immunocytochemistry staining of cultured cell lines4.
Application References(PubMed link indicates BioLegend citation): Allport JR, et al. 2002. J. Leukoc. Biol. 71:821. (IP WB) Hirashima M, et al. 2009. Blood 93:1253. (Block) Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. (Block) Matsumura K, et al. 2003. Blood 101:1367. (IF) Hirashima M, et al. 2009. Blood 101:2261. (FC) Urbschat A, et al. 2014. PLoS One. 9:84432. PubMed
Product Citations: Brulois K, et al. 2020. Nat Commun. 3.095833333. PubMed Li GY, et al. 2023. J Pharm Anal. 13:262. PubMed Fischer D, et al. 2018. Front Pharmacol. 8:E10. PubMed Wang JC, et al. 2019. J Exp Clin Cancer Res. 38:235. PubMed Urbschat A, et al. 2014. PLoS One. 9:84432. PubMed Khaiboullina S, et al. 2019. Front Pharmacol. 10:642. PubMed
RRID: AB_10569116 (BioLegend Cat. No. 138101)
Structure: A 120 kD member of type II Cadherin family
Distribution: Endothelial cells
Function: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion.
Cell Type: Endothelial cells, Mesenchymal Stem Cells
Biology Area: Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Gotsch U, et al. 1997. J. Cell Sci. 110:583. 2. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.
Gene ID: 12562
UniProt: View information about CD144 on UniProt.org
Clone: VECD1
Regulatory Status: RUO
Other Names: Vascular endothelial-cadherin, VE-cadherin
Isotype: Rat IgG1, κ