Biolegend, 138427, Brilliant Violet 711™ anti-mouse/human KLRG1 (MAFA) Antibody, 50μg

Killer cell lectin-like receptor G1 (KLRG1) is the mouse homolog of the rat mast cell function-associated antigen (MAFA or 2F1-Ag).  KLRG1 is a type II membrane glycoprotein that was first identified on the surface of rat mast cell line RBL-2H3.  It is composed of a homodimer of glycosylated 30-38 kD subunits.  Mouse and human homologs of KLRG1 are expressed by subsets of NK cells and lymphokine-activated killer (LAK) cells but not mast cells.  KLRG1 is also expressed on subsets of CD8 + and CD4 + cells, including CD4 + and CD8 + effector/memory cells, potent regulatory CD4 + T cells.  KLRG1 may be involved in regulating NK cell homeostasis.  KLRG1 was found to recognize cadherins and thus inhibit immune responses by regulating the effector function and the developmental processes of NK and T cells.
50μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Immunogen: IL-2 activated NK cells from C57BL/6 mice
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421™, or APC, are recommended.Additional reported applications (for relevant formats) include: Western Blotting1.
Application References(PubMed link indicates BioLegend citation): Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB) Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC) McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
Product Citations: Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed Backlund C, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204078119. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Clemmensen HS, et al. 2021. MBio. 12:. PubMed Clemmensen HS, et al. 2020. Front Immunol. 11:585359. PubMed Bachem A, et al. 2020. Immunity. 51(2):285-297. PubMed Beura LK, et al. 2018. Immunity. 48:327. PubMed Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
RRID: AB_2629721 (BioLegend Cat. No. 138427)
Disclaimer: This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Structure: KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.
Distribution: Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8+ and CD4+ cells.
Function: Regulates NK cell proliferation, maturation, and homeostasis. Inhibits immune responses by regulating the effector function and the developmental processes of NK and T cells.
Ligand/Receptor: E-cadherin
Cell Type: NK cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Grundemann C, et al. 2006. J. Immunol. 176:1311. 2. Blaser C, et al. 1998. J. Immunol. 161:6451. 3. Huntington ND, et al. 2007. J. Immunol. 178:4764. 4. Voehringer D, et al. 2001. J. Immunol. 167:4834.
Gene ID: 50928
UniProt: View information about KLRG1 on UniProt.org
Clone: 2F1/KLRG1
Regulatory Status: RUO
Other Names: MAFA, 2F1-Ag
Isotype: Syrian Hamster IgG