Biolegend, 138503, PE anti-mouse CD24 Antibody, 50μg

CD24 is a 35-45 kD protein, also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells, hematopoietic stem cells, and neurons. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus/spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (FC)
Product Citations: Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed Ruan X, et al. 2023. Nat Commun. 14:3275. PubMed Varshney R, et al. 2023. iScience. 26:105750. PubMed Bommireddy R, et al. 2020. Vaccines (Basel). 8:. PubMed Perry JSA, et al. 2018. Immunity. 48:923. PubMed Cong M, et al. 2021. Methods Mol Biol. 2388:59. PubMed Upadhya R, et al. 2016. MBio. 7: 00547-16. PubMed Bradley T et al. 2018. Cell reports. 25(1):107-117 . PubMed Kishi Y, et al. 2021. STAR Protocols. 2(2):100540. PubMed Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
RRID: AB_10576359 (BioLegend Cat. No. 138503) AB_10578416 (BioLegend Cat. No. 138504)
Structure: GPI-linked sialoglycoprotein, 35-45 kD.
Distribution: Lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells, and neurons
Function: Regulate B and T cell proliferation and differentiation.
Ligand/Receptor: P-selectin, CD24
Cell Type: B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Lymphocytes, Monocytes, Neurons, Thymocytes
Biology Area: Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Li YS, et al. 1993. J. Exp. Med. 178:951. 2. Aigner S, et al. 1995. Int. Immunol. 7:1557. 3. Hunte BE, et al. 1998. Eur. J. Immunol.. 28:3850. 4. Auerbach R, et al. 1996. Stem Cells 14:269. 5. Allman DM, et al. 1993. J. Immunol.. 151:4431.
Gene ID: 12484
UniProt: View information about CD24 on UniProt.org
Clone: 30-F1
Regulatory Status: RUO
Other Names: Heat Stable Antigen (HSA), HsAg, Ly-52, Nectadrin
Isotype: Rat IgG2c, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.