Biolegend, 139334, APC/Fire™ 750 anti-mouse CD64 (FcγRI) Antibody, 100μg

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
Application References(PubMed link indicates BioLegend citation): Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID: AB_2910296 (BioLegend Cat. No. 139333) AB_2910296 (BioLegend Cat. No. 139334)
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, mast cells, dendritic cells
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG
Cell Type: Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Gene ID: 14129
UniProt: View information about CD64 on UniProt.org
Clone: X54-5/7.1
Regulatory Status: RUO
Other Names: FcRI
Isotype: Mouse IgG1, κ