Biolegend, 140417, PerCP/Cyanine5.5 anti-mouse CD8b.2 Antibody, 25μg

CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.
Application References(PubMed link indicates BioLegend citation): Ledbetter J, et al. 1979. Immunol. Rev. 47:63. Ledbetter J, et al. 1980. J. Exp. Med. 152:280. Vremec D, et al. 2000. J. Immunol. 164:2978. (IF) Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC) Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)
Product Citations: Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed Elliot TAE, et al. 2022. Discov Immunol. 1:kyac009. PubMed Yang N, et al. 2022. NPJ Vaccines. 7:120. PubMed Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
RRID: AB_2800650 (BioLegend Cat. No. 140417) AB_2800651 (BioLegend Cat. No. 140418)
Structure: Ig superfamily, CD8β chain, 32 kD
Distribution: Majority of thymocytes, cytotoxic T cell subsets
Function: Co-receptor of TCR
Ligand/Receptor: MHC class I
Cell Type: T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503. 2. Renard V, et al. 1996. J. Exp. Med. 184:2439.
Gene ID: 12526
UniProt: View information about CD8beta.2 on UniProt.org
Clone: 53-5.8
Regulatory Status: RUO
Other Names: Lyt-3.2, Ly-3.2
Isotype: Rat IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.