Biolegend, 141103, FITC anti-mouse TCR Vγ1.1/Cr4 Antibody, 25μg

T cell receptor (TCR) is a heterodimer consisting of an α and β chain (TCR α/β) or a γ and δ chain (TCR γ/δ). TCR associates with CD3 to form a CD3/TCR complex. The CD3/TCR plays a key role in antigen recognition, signal transduction, and T cell activation. TCR Vγ1.1 (Garman nomenclature) is also called TCR Vγ1 (Tonegawa nomenclature). The Vγ1 gene almost exclusively rearranges to the Jγ4-Cγ4 gene. Vγ1- Jγ4-Cγ4 expressing cells constitute a major population of γ/δ T cells in the thymus and peripheral lymphoid organs in adult mice, but they are only composed of a minor population of γ/δ T cells during fetal and early postnatal life. Vγ1 T cell development can happen in thymus-dependent and thymus-independent manners. Further studies have shown that the antibody 2.11 recognizes an epitope located in the Cr4 domain. Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts .
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: T3.13.1 T-cell hybridoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Pereira P, et al. 1995. J. Exp. Med. 182:1921. (IP) Pereira P and Boucontet L. 2004. J. Immunol. 173:3261. (FC) Pereira P, et al. 1996. Intl. Immunol. 8:83. (FC)
Product Citations: Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed Fujimori S, et al. 2022. Elife. 11:. PubMed Ren S, et al. 2021. Front Immunol. 12:623451. PubMed Du Y, et al. 2022. Nat Commun. 13:231. PubMed Jin C, et al. 2019. Cell. 176:998. PubMed Guo Y, et al. 2013. Infect Immun . 81:3923. PubMed Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed Yi P, et al. 2017. Sci Rep. 2.970138889. PubMed Jaffar Z, et al. 2011. J Immunol. 187:5380. PubMed
RRID: AB_10694242 (BioLegend Cat. No. 141103) AB_10697031 (BioLegend Cat. No. 141104)
Structure: Ig superfamily
Distribution: Subset of γ/δ T-cells
Function: Antigen recognition
Ligand/Receptor: Antigen
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Pereira P, et al. 1995. J. Exp. Med. 182:1921. 2. Grigoriadou K, et al. 2002. J. Immunol. 169:3736.
Gene ID: 110067
UniProt: View information about TCR Vgamma1.1/Cr4 on UniProt.org
Clone: 2.11
Regulatory Status: RUO
Other Names: T cell receptor Vgamma 1.1, TCR Vγ1 (Tonegawa nomenclature), TCR Cr4, TRGV1, GV5S1
Isotype: Armenian Hamster IgG