Biolegend, 141701, Purified anti-mouse CD206 (MMR) Antibody, 50μg

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD206 (MMR)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested FC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
Application References(PubMed link indicates BioLegend citation): Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed Yang X, et al. 2015. PNAS. 112:2900. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed Li H, et al. 2022. Cancer Cell. 40:36. PubMed Scott EP, et al. 2022. Glia. 70:1720. PubMed Loyher PL, et al. 2018. J Exp Med. 215:2536. PubMed Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed Wang W, et al. 2018. Cancer Cell. 34:757. PubMed Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed Zappasodi R, et al. 2021. Nature. 591:652. PubMed Nolin JD, et al. 2017. JCI Insight. 2:e94929. PubMed Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed Bisht K, et al. 2021. Nat Commun. 12:5289. PubMed Sun KT, et al. 2018. Front Physiol. 1.06875. PubMed Hu G, et al. 2021. Nat Commun. 12:773. PubMed Enders M, et al. 2020. J Immunol. 204:87. PubMed Tian Z, et al. 2022. Front Integr Neurosci. 15:742377. PubMed Blriot C, et al. 2021. Immunity. :. PubMed Dong Y, et al. 2020. Front Immunol. 11:29. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed Yang X, et al. 2015. Proc Natl Acad Sci U S A. 112:2900. PubMed Kim D, et al. 2019. Immune Netw. 19:e32. PubMed Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed Wang G, et al. 2019. Front Immunol. 1.380555556. PubMed Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
RRID: AB_10900263 (BioLegend Cat. No. 141701) AB_10900233 (BioLegend Cat. No. 141702)
Structure: Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution: Macrophages, dendritic cells, Langerhans cells, liver endothelial cells
Function: Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor: Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type: Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: CD Molecules
Antigen References: 1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501. 2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469. 3. Burgdorf S, et al. 2006. J. Immunol. 176:6770. 4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.
Gene ID: 17533
UniProt: View information about CD206 on UniProt.org
Clone: C068C2
Regulatory Status: RUO
Other Names: MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype: Rat IgG2a, κ
Q: Why is mouse CD206 stained intracellularly and not via surface staining?
A: Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.