Biolegend, 142213, PerCP/Cyanine5.5 anti-mouse CD200R3 Antibody, 25μg

CD200R3, also known as CD200RLb and OX-2 Receptor 3, is a disulfide-linked dimeric CD200R-like receptor which belongs to immunoglobulin superfamily.  Its positively charged amino acid lysine associates with ITAM- or YxxM motif-bearing adaptor molecules such as DAP12, DAP10, FcRγ, and CD3ζ. CD200R3 functions as an activating receptor to regulate IgE independent immune response.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse primary basophils
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Ba13 recognizes circulating and bone marrow basophils; it also recognizes a subset of mast cells in the peritoneal cavity and skin. Additional reported applications (for the relevant formats) include: stimulation of bone marrow derived basophils to produce IL-4.
Product Citations: Xiao X, et al. 2024. J Cell Physiol. e31181:239. PubMed
RRID: AB_2810409 (BioLegend Cat. No. 142213) AB_2810410 (BioLegend Cat. No. 142214)
Structure: Immunoglobulin superfamily; disulfide-linked dimer with positively charge amino acids in transmembrane; molecular weight is about ~38 kD or ~74 kD (reduced or non-reduced conditions, respectively)
Distribution: Basophils in peripheral blood, bone marrow, spleen; mast cells in peritoneal cavity and skin
Function: Transduce stimulatory signals
Interaction: DAP12, DAP10, FcRγ, or CD3ζ
Cell Type: Basophils, Mast cells
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Voehringer D, et al. 2004. J. Biol. Chem. 52:54117. 2. Kojima T, et al. 2007. J. Immunol. 179:7093. 3. Sato K, et al. 2009. Blood 113:4780.
Gene ID: 74603
UniProt: View information about CD200R3 on UniProt.org
Clone: Ba13
Regulatory Status: RUO
Other Names: CD200 R3, CD200RLb, OX-2 Receptor 3, CD200 receptor 3
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.