Biolegend, 143403, PE anti-mouse CD371 (CLEC12A) Antibody, 25μg

CD371 (CLEC12A), also known as DCAL-2, MICL or CLL-1, is a 30 kD type II transmembrane protein with extracellular C-type lectin domains, belonging to the C-type lectin family.  Its cytoplasmic ITIM motif modulates signaling cascades and is involved in phosphorylation of tyrosine residues in MAP kinases. Mouse CLEC12A is expressed on CD8 + dendritic cells (DCs), a proportion of CD8 - dendritc cells, plasmacytoid DCs, monoctyes, macrophages, granulocytes, and B cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO expressing mouse CLEC12A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Raulf MK, et al. 2019. Cell Rep. 28:30. PubMed Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
RRID: AB_11126747 (BioLegend Cat. No. 143403) AB_11126984 (BioLegend Cat. No. 143404)
Structure: Type II C-type lectin family member, 265 aa, 30.7 kD
Distribution: CD8+ dendritic cells (DCs), a proportion of CD8- dendritic cells, plasmacytoid DCs, monocytes, macrophages, granulocytes, B cells
Function: Negative regulator of granuocyte and monocyte function
Interaction: PTPN6 and PTPN11
Cell Type: B cells, Dendritic cells, Embryonic Stem Cells, Granulocytes, Macrophages, Monocytes
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Lahoud MH, et al. 2009. J. Immunol. 182:7587. 2. Chen CH, et al. 2006. Blood 107:1459. 3. Marshall AS, et al. 2004. J. Biol. Chem. 279:14792.
Gene ID: 232413
UniProt: View information about CD371 on UniProt.org
Clone: 5D3/CLEC12A
Regulatory Status: RUO
Other Names: CLL1, MICL, CLL-1, DCAL-2, C-type lectin domain family 12 member A, CD371
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.