Biolegend, 144204, PE anti-mouse/human CD207 (Langerin) Antibody, 100μg

CD207, also known as langerin, is a 40 kD single-pass type II transmembrane protein, member of the C-type lectin family. CD207 is expressed on the cell surface and Birbeck granules (BGs) of Langerhans cells, and subsets of thymic and splenic dendritic cells. Its ligands are mannose, n -acetylglucosamine, fucose, and sulfated glycans. Langerin is involved in the antigen processing pathway through capture and internalization of its ligands.
100μg
Verified Reactivity: Mouse, Human
Reported Reactivity: Cynomolgus, Rabbit, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Langerin extracellular domain-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality testedIHC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Huang T, et al. 2023. Cell Death Dis. 14:354. PubMed Zhou H, et al. 2022. EMBO Rep. 23:e53791. PubMed Hardman CS, et al. 2022. Nat Commun. 13:7535. PubMed Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed Li Z, et al. 2021. Immunology. 163:105. PubMed Park S, et al. 2021. Nat Cell Biol. 23:476. PubMed Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed Acker KP, et al. 2019. iScience. 19:281. PubMed Zhang X, et al. 2022. JCI Insight. 7:. PubMed Sheng J, et al. 2021. eLife. 10:00. PubMed Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
RRID: AB_2561498 (BioLegend Cat. No. 144203) AB_2561499 (BioLegend Cat. No. 144204)
Structure: Single-pass type II transmembrane protein, 40 kD, one C-type lectin domain, homotrimer
Distribution: Langerhans cells, subsets of thymic and splenic dendritic cells
Function: Antigen uptake, antigen processing
Ligand/Receptor: Mannose, n-acetylglucosamine, fucose, sulfated glycans
Cell Type: Dendritic cells, Langerhans cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Romani N, et al. 2012. Curr. Top. Microbiol. Immunol. 351:113. 2. Kaplan DH. 2010. Trends Immunol. 31:446. 3. Clausen BE and Kel JM. 2010. Immunol. Cell. Biol. 88:351. 4. Merad M, et al. 2008. Nat. Rev. Immunol. 8:935.
Gene ID: 24627850489
UniProt: View information about CD207 on UniProt.org
Clone: 4C7
Regulatory Status: RUO
Other Names: CLEC4K
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.