Biolegend, 145208, PerCP/Cyanine5.5 anti-mouse CD304 (Neuropilin-1) Antibody, 100μg

CD304, also known as neuropilin-1, is a 140 kD type I transmembrane protein. Its extracellular region contains two CUB, two FV/FVIII, and one MAM domain. It is expressed by natural regulatory T cells (nTreg), a subset of invariant natural killer T cells (iNKT), endothelial cells, and neurons. Neuropilin-1 stabilizes the interaction between Tregs and dendritic cells, contributes to the recruitment of tumor-infiltrating Tregs in response to tumor-derived VEGF, and influences the process of angiogenesis and axon guidance. The ligands of CD304 are VEGF165 and semaphorin-3A.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Extracellular region of mouse CD304
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed Verhagen J, et al. 2014. PLoS One. 9e:108023. (FC) PubMed
Product Citations: Stathopoulou C, et al. 2020. Immunity. 49(2):247-263.e7.. PubMed Young JS, et al. 2018. Front Immunol. 9:1385. PubMed
RRID: AB_2562033 (BioLegend Cat. No. 145207) AB_2562034 (BioLegend Cat. No. 145208)
Structure: Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
Distribution: Natural regulatory T cells (nTreg), subset of invariant natural killer T cells (iNKT), endothelial cells, neurons
Function: Angiogenesis, axon guidance, modulation of the immune response
Ligand/Receptor: VEGF165, semaphorin-3A
Cell Type: Dendritic cells, Endothelial cells, Neurons, NKT cells, Tregs
Biology Area: Angiogenesis, Cancer Biomarkers, Cardiovascular Biology, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family: Adaptor Proteins, Adhesion Molecules, CD Molecules
Antigen References: 1. Yadav M, et al. 2012. J. Exp. Med. 209:1713. 2. Weiss JM, et al. 2012. J. Exp. Med. 209:1723. 3. Hansen W, et al. 2012. J. Exp. Med. 209:2001. 4. Milpied P, et al. 2011. Blood 118:2993.
Gene ID: 18186
UniProt: View information about CD304 on UniProt.org
Clone: 3E12
Regulatory Status: RUO
Other Names: NRP-1, VEGFR165 receptor
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.