Biolegend, 145311, PerCP/Cyanine5.5 anti-mouse IL-33Rα (IL1RL1, ST2) Antibody, 25μg

IL-33Rα, also known as ST2 or IL-1RL1, is a member of the Toll/IL-1 receptor family.  It binds IL-33 and is structurally similar to IL-1R1.  Two forms of the protein exist - a soluble form known as ST2 and a membrane anchored form known as ST2L.  The membrane form is expressed by Th2 cells and bone marrow derived mast cells, whereas the soluble form is expressed by serum-stimulated fibroblasts. Blocking with anti-ST2 antibodies has been shown to alleviate experimental arthritis and airway inflammation.  The IL-33-ST2 axis has been reported to be important across a range of diseases including asthma, allergies, and cardiac disease.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-33Rα-hFcγ1 fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Hashiguchi M, et al. 2014. Eur. J. Immunology. (FC) PubMed
Product Citations: Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed de Lucía Finkel P, et al. 2021. Sci Rep. 11:12233. PubMed Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
RRID: AB_2565635 (BioLegend Cat. No. 145311) AB_2565636 (BioLegend Cat. No. 145312)
Structure: 63 kD, IL-1 receptor family member, single pass type I membrane protein
Distribution: Mast cells, Th2 cells, and fibroblasts
Function: Following interaction with IL-33, it recruits MyD88, Irak1, Irak4 and Traf6 followed by phosphorylation of the MAP/ERK pathway.
Interaction: Interacts with MyD88, Irak1, Irak4 and Traf6
Ligand/Receptor: IL-33
Cell Type: Fibroblasts, Mast cells, Th2
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: 1. Yanagisawa K, et al. 1993. FEBS Lett. 318:83. 2. Schmitt E, et al. 1990. Cytokine 6:407. 3. Yanagisawa K, et al. 1992. FEBS Lett. 302:51. 4. Takagi T, et al. 1993. Biochim Biophys Acta. 1178:194.
Gene ID: 17082
UniProt: View information about IL-33Ralpha on UniProt.org
Clone: DIH9
Regulatory Status: RUO
Other Names: T1/ST2, ST2L, IL-1R1, T1, DER4, IL1R1
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.