Biolegend, 145504, PE anti-mouse CD185 (CXCR5) Antibody, 100μg

CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: mCXCR5-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.
Product Citations: del Rio ML, et al. 2023. Front Immunol. 14:1113858. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Rios D, et al. 2015. Mucosal Immunol. 101038/mi. PubMed Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed Xing Y, et al. 2022. Cancer Lett. 529:100. PubMed Frost JN, et al. 2021. Med (N Y). 2:164. PubMed Tong D, et al. 2020. Protein Cell. 0.533333333. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
RRID: AB_2561967 (BioLegend Cat. No. 145503) AB_2561968 (BioLegend Cat. No. 145504)
Structure: 42 kD, seven transmembrane protein
Distribution: B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain
Function: B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
Ligand/Receptor: BLC/CXCL13
Cell Type: B cells, T cells, Tfh
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532. 2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381. 3. Forster R, et al. 1994. Blood 84:830. 4. Forster R, et al. 1996. Cell 87:1037. 5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.
Gene ID: 12145
UniProt: View information about CD185 on UniProt.org
Clone: L138D7
Regulatory Status: RUO
Other Names: BLR1, GPCR6, MDR-15
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.