Biolegend, 146104, PE anti-mouse CLEC-2 (CLEC1B) Antibody, 100μg

CLEC-2, also known as CLEC1B and CLEC1B1, is C-type lectin-like receptor with a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM). CLEC-2 is highly expressed on resting platelets and megakaryocytes and at a lower levels on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. The first ligand that was identified for CLEC-2 was the snake venom toxin rhodocytin; podoplanin has also been identified as an endogenous ligand for CLEC-2. Exposure of platelets to rhodocytin leads tyrosine phosphorylation of the ITAM-like motif and Syk-dependent platelet activation. CLEC-2 also functions as an activation receptor on monoyctes and neutrophils to induce phagocytosis and proinflammatory cytokine production.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: RBL-2H3 cells stably expressing HA-tagged mouse CLEC2.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for relevant formats) include: Western blotting1.
Application References(PubMed link indicates BioLegend citation): Mourpo-Sß D, et al. 2011. Eur. J. Immunol. 41:3040. (WB)
Product Citations: Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed Nording H, et al. 2021. Nat Commun. 12:3352. PubMed Sung PS, et al. 2019. Nat Commun. 10:2402. PubMed Tang C, et al. 2021. Theranostics. 11:9791. PubMed Daemen S, et al. 2021. STAR Protoc. 2:100511. PubMed Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
RRID: AB_2562382 (BioLegend Cat. No. 146103) AB_2562383 (BioLegend Cat. No. 146104)
Structure: Type II transmembrane protein with a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM)
Distribution: Platelets, megakaryocytes, granulocytes, monocytes, macrophages, and dendritic cells
Function: Involved in platelet aggregation and secretion as well as neutrophil phagocytosis
Ligand/Receptor: Venom toxin, rhodocytin, and podoplanin
Cell Type: Dendritic cells, Granulocytes, Macrophages, Megakaryocytes, Monocytes, Platelets, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References: 1. Boulaftali Y, et al. 2013. J. Clin. Invest. 123:908. 2. Manne BK, et al. 2013. J. Biol. Chem. 288:7717. 3. Acton SE, et al. 2012. Immunity. 37:276. 4. Finney BA, et al. 2012. Blood 119:1747. 5. Kerrigan AM, et al. 2009. J. Immunol. 182:4150.
Gene ID: 56760
UniProt: View information about CLEC-2 on UniProt.org
Clone: 17D9/CLEC-2
Regulatory Status: RUO
Other Names: CLEC1B, CLEC1B1
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.