Biolegend, 148105, APC anti-mouse CD285 (TLR5) Antibody, 25μg

CD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: TLR5-transfected Ba/F3 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications for the relevant formats include: immunoprecipitation of TLR5 from cell lysates1,2.
Application References(PubMed link indicates BioLegend citation): Shibata T, et al. Int Immunol. 24:613. (IP, FC) Huh JW, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:7072. (IP)
Product Citations: Lee GR, et al. 2021. JCI Insight. 6:. PubMed
RRID: AB_2890695 (BioLegend Cat. No. 148105) AB_2890695 (BioLegend Cat. No. 148106)
Structure: Type 1 transmembrane protein, 98 kD
Distribution: Neutrophils, CD11b, Ly6C, classical monocytes, dendritic cells, mucosal epithelium
Interaction: Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
Ligand/Receptor: Flagellin
Cell Type: Dendritic cells, Monocytes, Neutrophils
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. López-Yglesias AH, et al. 2014. J. Immunol. 192:1587. 2. Burdelya L, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E1857. 3. Carapau D, et al. 2013. Infect. Immun. 81:4350. 4. Kinnebrew M, et al. 2012. Immunity. 36:276. 5. Sebastiani G, et al. 2000. Genomics. 64:230.
Gene ID: 53791
UniProt: View information about CD285 on UniProt.org
Clone: ACT5
Regulatory Status: RUO
Other Names: Toll-like receptor 5, TIL3, SLEB1, MELIOS
Isotype: Mouse IgG1, κ