Biolegend, 148220, Brilliant Violet 650™ anti-mouse/rat XCR1 Antibody, 50μg

XCR1, also known as GPR5 or CCXCR1, is a 38 kD, G-protein coupled, seven transmembrane receptor, and the only member of the "C" chemokine receptor family. XCR1 mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2), and defines a subset of CD8α + conventional dendritic cells capable of antigen cross-presentation.
50μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.
Application References(PubMed link indicates BioLegend citation): Gurka S, et al. 2015. Front Immunol. 6:35. (FC) Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
Product Citations: Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Cansever D, et al. 2023. Nat Immunol. . PubMed Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Klaus T, et al. 2022. JCI Insight. 7: . PubMed Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed Wiernicki B, et al. 2022. Nat Commun. 13:3676. PubMed Rao E, et al. 2021. Sci Immunol. 6:. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Marangoni F, et al. 2021. Cell. . PubMed Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed Guilliams M, et al. 2022. Cell. 185:379. PubMed Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed Remmerie A, et al. 2020. Immunity. 53:641. PubMed Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed Zhang L, et al. 2020. Cell. 442:181. PubMed Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed Goc J, et al. 2021. Cell. 184:5015. PubMed Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
RRID: AB_2566410 (BioLegend Cat. No. 148220)
Structure: Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
Distribution: Subset of CD8α+ conventional dendritic cells.
Function: Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
Ligand/Receptor: XCL1 and XCL2.
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yamazaki C, et al. 2013. J. Immunol. 190:6071. 2. Shimizu K, et al. 2013. J. Immunol. 190:5609. 3. Bachem A, et al. 2012. Front. Immunol. 3:214. 4. Crozat K, et al. 2011. J. Immunol. 187:4411.
Gene ID: 23832301086
UniProt: View information about XCR1 on UniProt.org
Clone: ZET
Regulatory Status: RUO
Other Names: GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Isotype: Mouse IgG2b, κ