Biolegend, 149104, PE anti-mouse CCL5 (RANTES) Antibody, 100μg

Chemokine (C-C motif) ligand 5 (CCL5), also known as regulated on activation, normal T cell expressed and secreted (RANTES), is a 10 kD protein primarily produced by CD8 + T cells. CCL5 can also be secreted by platelets, dendritic cells (DCs), and fibroblasts. CCL5 functions as a chemoattractant for T cells, monocytes, and eosinophils through binding of its receptor, CCR5. CCL5 can inhibit binding of HIV-1 to CCR5 and inhibit HIV-1 entry.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant CCL5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Jubin V, et al. 2012. Immunol. Res. 52:284.
Product Citations: Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed Tian L, et al. 2022. Nat Cancer. 3:1318. PubMed Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed Böttcher JP, et al. 2018. Cell. 172:1022. PubMed Mamedov MR, et al. 2018. Immunity. 48:350. PubMed Todorov H, et al. 2022. iScience. 25:104927. PubMed Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed Collins N, et al. 2016. Nat Commun. 7:11514. PubMed Kim SJ, et al. 2020. Cell Mol Immunol. 17:728. PubMed Sung PS, et al. 2022. JCI Insight. :. PubMed
RRID: AB_2564405 (BioLegend Cat. No. 149103) AB_2564406 (BioLegend Cat. No. 149104)
Structure: Chemokine (C-C motif) ligand 5, 10 kD
Distribution: CD8+ T cells, platelets, DCs, and fibroblasts.
Function: Chemoattractant for T cells, monocytes, and eosinophils.
Ligand/Receptor: CCR5, CCR3, and CCR1.
Cell Type: Dendritic cells, Monocytes, Macrophages, Granulocytes, B cells
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Schall TJ. 1991. Cytokine 3:165. 2. Schall TJ, et al. 1992. Eur. J. Immunol. 6:1477. 3. Son MH, et al. 2014. Biochem. Biophys. Res. Commun. 450:30.
Gene ID: 20304
UniProt: View information about CCL5 on UniProt.org
Clone: 2E9/CCL5
Regulatory Status: RUO
Other Names: CCL5, SISd, Scya5, RANTES, TCP228
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.