Biolegend, 149303, PE anti-mouse CD182 (CXCR2) Antibody, 25μg

CD182, also known as CXCR2, is a G-protein coupled receptor with 7 transmembrane regions that is involved in chemotaxis, neutrophil activation and angiogenesis. CXCR2 is expressed by neutrophils, basophils, subset of T cells, monocytes, macrophages, NK and NKT cells. Its ligands are CXCL1, CXCL2, CXCL3 and CXCL5.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CXCR2-transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Barkaway A, et al. 2021. Immunity. . PubMed Feng X, et al. 2023. Nat Commun. 14:3208. PubMed Li Z, et al. 2022. Elife. 11:. PubMed Kulkarni U, et al. 2019. Mucosal Immunol. 12:545. PubMed Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed Lin X, et al. 2022. Front Mol Biosci. 9:806528. PubMed Engblom C, et al. 2017. Science. 358:6367. PubMed Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
RRID: AB_2565691 (BioLegend Cat. No. 149303) AB_2565692 (BioLegend Cat. No. 149304)
Structure: G-protein coupled receptor, 7 transmembrane regions.
Distribution: Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer cells (NK).
Function: Chemotaxis, neutrophil activation, and angiogenesis
Ligand/Receptor: CXCL1, CXCL2, CXCL3, and CXCL5.
Cell Type: Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Spaan AN, et al. 2014. Nat. Commun. 5:5438. 2. Natsuaki Y, et al. 2014. Nat. Immunol. 11:1064. 3. Hsieh CY, et al. 2014. J. Immunol. 193:3693. 4. Diana J, and Lehuen A. 2014. EMBO Mol. Med. 6:1090. 5. Stoolman JS, et al. 2014. J. Immunol. 193:564.
Gene ID: 12765
UniProt: View information about CD182 on UniProt.org
Clone: SA044G4
Regulatory Status: RUO
Other Names: IL-8RB, GRO/MGSA, Cmkar2
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.