Biolegend, 149605, PerCP/Cyanine5.5 anti-mouse CD182 (CXCR2) Antibody, 25μg

CD182, also known as CXCR2, is a G-protein coupled receptor with seven transmembrane regions that is involved in chemotaxis, neutrophil activation, and angiogenesis. CXCR2 is expressed by neutrophils, basophils, a subset of T cells, monocytes, macrophages, natural killer (NK) and natural killer T cells (NKT). Its ligands are CXCL1, CXCL2, CXCL3, and CXCL5.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CXCR2-transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Caxaria S, et al. 2023. Proc Natl Acad Sci U S A. e2211631120:120. PubMed Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
RRID: AB_2565564 (BioLegend Cat. No. 149605) AB_2565565 (BioLegend Cat. No. 149606)
Structure: G-protein coupled receptor and seven transmembrane regions.
Distribution: Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer (NK) cells.
Function: Chemotaxis, neutrophil activation, and angiogenesis.
Ligand/Receptor: CXCL1, CXCL2, CXCL3, and CXCL5.
Cell Type: Basophils, Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Spaan AN, et al. 2014. Nat. Commun. 5:5438. 2. Natsuaki Y, et al. 2014. Nat. Immunol. 15:1064. 3. Hsieh CY, et al. 2014. J. Immunol. 193:3693. 4. Diana J and Lehuen A. 2014. EMBO Mol. Med. 6:1090. 5. Stoolman JS, et al. 2014. J. Immunol. 193:564.
Gene ID: 12765
UniProt: View information about CD182 on UniProt.org
Clone: SA045E1
Regulatory Status: RUO
Other Names: IL-8RB, GRO/MGSA, Cmkar2
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.