Biolegend, 150005, PE anti-mouse Blimp-1 Antibody, 25μg

Blimp-1, also known as PRDM1, is a 98 kD protein containing 5 Kruppel-type zinc finger domains. Blimp-1 represses the transcription factors BCL6 and c-Myc. It is the master regulator of terminal B cell differentiation and is also involved in the differentiation and homeostasis of T cells and natural killer (NK) cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Amino acids 255-395 from mouse Blimp-1 fused with GST.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Product Citations: Chen HY, et al. 2022. Elife. 11:. PubMed Xu Y, et al. 2022. Nat Commun. 13:980. PubMed Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
RRID: AB_2565991 (BioLegend Cat. No. 150005) AB_2565992 (BioLegend Cat. No. 150006)
Structure: Five Kruppel-type zinc finger domains, 98 kD.
Distribution: Plasma cells, CD4+ and CD8+ effector/memory T cells, and natural killer cells.
Function: Terminal differentiation of B cells, effector/memory T cells, role in natural killer cells (NK) and T cell homeostasis, and repressor of BCL6 and c-Myc.
Cell Type: Plasma cells, T cells, NK cells, B cells
Biology Area: Cell Biology, Immunology, Transcription Factors
Antigen References: 1. Nakaki F, et al. 2013. Nature 501:222. 2. Crotty S, et al. 2010. Nat. Immunol. 11:114. 3. Zhao WL, et al. 2008. Blood 111:3867. 4. Climmino L, et al. 2008. J. Immunol. 181:2338. 5. Martins, G and Clarne, K. 2008. Annu. Rev. Immunol. 26:133.
Gene ID: 12142
UniProt: View information about Blimp-1 on UniProt.org
Clone: 5E7
Regulatory Status: RUO
Other Names: PR domain zinc finger protein 1, B lymphocyte-induced maturation protein-1, PRDM1, BLIMP1, PRDI-BF1, ZNFPR1A1, PRDM-1, BLIMP1
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.