Biolegend, 150009, Brilliant Violet 421™ anti-mouse Blimp-1 Antibody, 50μg

Blimp-1, also known as PRDM1, is a 98 kD protein containing 5 Kruppel-type zinc finger domains. Blimp-1 represses the transcription factors BCL6 and c-Myc. It is the master regulator of terminal B cell differentiation and is also involved in the differentiation and homeostasis of T cells and natural killer (NK) cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Amino acids 255-395 from mouse Blimp-1 fused with GST.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
RRID: AB_3683165 (BioLegend Cat. No. 150009)
Structure: Five Kruppel-type zinc finger domains, 98 kD.
Distribution: Plasma cells, CD4+ and CD8+ effector/memory T cells, and natural killer cells.
Function: Terminal differentiation of B cells, effector/memory T cells, role in natural killer cells (NK) and T cell homeostasis, and repressor of BCL6 and c-Myc.
Cell Type: B cells, NK cells, Plasma cells, T cells
Biology Area: Cell Biology, Immunology, Transcription Factors
Antigen References: 1. Nakaki F, et al. 2013. Nature 501:222. 2. Crotty S, et al. 2010. Nat. Immunol. 11:114. 3. Zhao WL, et al. 2008. Blood 111:3867. 4. Climmino L, et al. 2008. J. Immunol. 181:2338. 5. Martins, G and Clarne, K. 2008. Annu. Rev. Immunol. 26:133.
Gene ID: 12142
UniProt: View information about Blimp-1 on UniProt.org
Clone: 5E7
Regulatory Status: RUO
Other Names: PR domain zinc finger protein 1, B lymphocyte-induced maturation protein-1, PRDM1, BLIMP1, PRDI-BF1, ZNFPR1A1, PRDM-1, BLIMP1
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.