Biolegend, 150438, APC/Fire™ 810 anti-mouse CD20 Antibody, 100μg

CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca 2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD20 - transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Red Laser (633 nm)
RRID: AB_2927876 (BioLegend Cat. No. 150437) AB_2927876 (BioLegend Cat. No. 150438)
Structure: Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
Distribution: B cells and a subset of T cells.
Function: Regulates B-cell activation and proliferation.
Interaction: Associated with MHC class I and II, CD53, CD81, and CD82.
Cell Type: B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Morsy DE, et al. 2013. J. Immunol. 191:3112. 2. Lund FE, Randall TD. 2010. Nat. Rev. Immunol. 10:236. 3. Beers SA, et al. 2010. Blood 115:5191. 4. Kuijpers TW, et al. 2010. J. Clin. Invest. 120:214.
Gene ID: 12482
UniProt: View information about CD20 on UniProt.org
Clone: SA275A11
Regulatory Status: RUO
Other Names: Ms4a1, Ly-44
Isotype: Rat IgG2b, κ