Biolegend, 150605, Brilliant Violet 421™ anti-mouse CD192 (CCR2) Antibody, 50μg

CD192, also known as CCR2, is a 42 kD G-protein coupled receptor that is associated with bone marrow homeostasis. Specifically, CD192 mediates monocyte chemotaxis and acts as a receptor for monocyte chemoattractant protein 1 (MCP-1). CD192 is primarily expressed on monocytes and macrophages, with some expression on basophils. It is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis and cancer.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CCR2 transfectants.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Product Citations: Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed Silva BM, et al. 2023. J Clin Invest. 133:. PubMed Edwards CL, et al. 2023. J Clin Invest. 133: . PubMed Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed Ferrere G, et al. 2021. JCI Insight. 6:. PubMed Yang F, et al. 2020. Cancer Res. 80:3677. PubMed Monaghan KL, et al. 2020. J Vis Exp. . PubMed Yao Y, et al. 2018. Cell. 175:1634. PubMed Jung SH, et al. 2022. Nat Commun. 13:4580. PubMed Wong NR, et al. 2021. Immunity. 54:2072. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Gong P, et al. 2022. Cell Death Discov. 8:466. PubMed Sharma R, et al. 2021. J Neuroinflammation. 72:18. PubMed Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
RRID: AB_2571913 (BioLegend Cat. No. 150605)
Structure: G-protein-coupled receptor, 7-transmembrane protein, 42 kD.
Distribution: Monocytes/macrophages and basophils.
Function: Mediator of monocyte chemotaxis and bone marrow homeostasis.
Ligand/Receptor: MCP-1, Ccl2, Ccl7, and Ccl13.
Cell Type: Basophils, Macrophages, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Mack M, et al. 2001. J. Immunol. 166:4697. 2. Dutta P, et al. 2015. Cell Stem Cell. 16:477. 3. Li L, et al. 2008. Kidney Int. 74:1526.
Gene ID: 12772
UniProt: View information about CD192 on UniProt.org
Clone: SA203G11
Regulatory Status: RUO
Other Names: Ccr2, Ckr2, Ccr2a, Ccr2b
Isotype: Rat IgG2b, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.