Biolegend, 151116, PE/Dazzle™ 594 anti-mouse CD186 (CXCR6) Antibody, 25μg

CD186, or CXCR6, is a 39 kD G-protein coupled chemokine receptor with seven transmembrane-spanning regions. Its ligand is CXCL16. It is expressed on different T cell subsets and is upregulated in activated T cells. Expression of CXCR6 is correlated with increased inflammatory responses and seems to contribute to liver fibrosis.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: mCXCR6-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Kedmi R, et al. 2022. Nature. 610:737. PubMed Zander R, et al. 2022. Elife. 11:. PubMed Lopes N, et al. 2022. Cell Rep Med. 3:100812. PubMed Anadon CM, et al. 2022. Cancer Cell. 40:545. PubMed du Halgouet A, et al. 2023. Immunity. 56:78. PubMed Zander R, et al. 2022. Immunity. 55:475. PubMed Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
RRID: AB_2721699 (BioLegend Cat. No. 151116) AB_2721700 (BioLegend Cat. No. 151117)
Structure: G-protein coupled receptor 1 family.
Distribution: T cells and natural killer T (NKT) cells.
Function: Chemotaxis and the development of T cells.
Ligand/Receptor: CXCL16.
Cell Type: NKT cells, T cells
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Kim CH, et al. 2001. J. Clin. Invest. 107:595. 2. Heesch K, et al. 2014. PLoS One. 9:5. 3. Wehr A, et al. 2013. J. Immunol. 190:5226.
Gene ID: 80901
UniProt: View information about CD186 on UniProt.org
Clone: SA051D1
Regulatory Status: RUO
Other Names: CXCR6, Chemokine (C-X-C motif) receptor 6, BONZO, STRL33, chemokine receptor 6, CD 186, CXC-R6, CXCR-6, C-X-C chemokine receptor type 6
Isotype: Rat IgG2b, κ