Biolegend, 151521, PE/Cyanine7 anti-mouse MERTK (Mer) Antibody, 25μg

MerTK (Mer) is a member of the TAM (TYRO3/AXL/MerTK) family. It is a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain. MerTK is mainly expressed by macrophages, monocytes, and dendritic cells. Its ligands are LGALS3, TUB, TULP1, and GAS6. MerTK is involved in the regulation of TLR signaling, efferocytosis, phagocytosis, cell survival, macrophage migration, and the inhibition of inflammation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse MERTK extracellular domain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Chang CY, et al. 2022. Sci Adv. 8:eabq0615. PubMed Pitzer Mutchler A, et al. 2023. Front Physiol. 14:1167904. PubMed
RRID: AB_2876508 (BioLegend Cat. No. 151521) AB_2876508 (BioLegend Cat. No. 151522)
Structure: Member of the TAM (TYRO3/AXL/MerTK) family. Encodes a transmembrane protein, two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain.
Distribution: Macrophages, monocytes, and dendritic cells.
Function: Regulates TLR signaling, efferocytosis, phagocytosis, cell survival, and macrophage migration. Inhibits production of inflammatory cytokines.
Interaction: Grb2, LimD4, and Vav1.
Ligand/Receptor: LGALS3, TUB, TULP1, and GAS6.
Cell Type: Dendritic cells, Macrophages, Monocytes
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Toll Like Receptors
Antigen References: 1. Zagórska A, et al. 2014. Nat. Immunol. 15:920. 2. Toda S, et al. 2014. Blood 123:3963. 3. Chung WS, et al. 2013. Nature 504:394. 4. Carrera Silva EA, et al. 2013. Immunity 39:160. 5. Yi Z, et al. 2009. Blood 114:3191.
Gene ID: 17289
UniProt: View information about MERTK on UniProt.org
Clone: 2B10C42
Regulatory Status: RUO
Other Names: Mer, Eyk, Nyk, nmf12
Isotype: Rat IgG2a, κ