Biolegend, 152609, PerCP/Cyanine5.5 anti-mouse CD95 (Fas) Antibody, 25μg

CD95, also known as Fas, is an approximately 45 kD type I transmembrane protein belonging to the TNFR superfamily (TNFRSF6). The expression of CD95 has been described in various organs, such as  thymus, spleen, liver, heart, lung and ovary. Upon ligand (FasL) binding, CD95 forms the death-inducing signaling complex (DISC) intracellularly and induce apoptosis of the cell. CD95-induced apoptosis plays important roles in development, as well as in maintining peripheral tolerance of the immune system.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse Fas Transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Rivas MA, et al. 2021. Nat Immunol. 22:240. PubMed Leung W, et al. 2022. Cancer Discov. 12:1782. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Tan L, et al. 2019. Cell Rep. 27:3657. PubMed Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed Lu X, et al. 2019. Cell Rep. 28:472. PubMed Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
RRID: AB_2632904 (BioLegend Cat. No. 152609) AB_2632905 (BioLegend Cat. No. 152610)
Structure: CD95 (Fas) is an approximately 45 kD type I transmembrane protein; cystiene-rich domain, death domain.
Distribution: Thymus, spleen, liver, heart, lung, and ovary.
Function: Induction of apoptosis.
Interaction: Caspase 8, caspase 10, CFLAR, FADD, PDCD6, SUMO1, and TNFRSF6.
Ligand/Receptor: FasL
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: Ogasawara J, et al. 1995. J Exp Med. 181:485-91. Ogasawara J, et al. 1993. Nature. 364:806-9. Nishimura Y, et al. 1995. J Immunol. 154:4395-403.
Gene ID: 14102
UniProt: View information about CD95 on UniProt.org
Clone: SA367H8
Regulatory Status: RUO
Other Names: Fas, ALPS1A, APO-1, APT1
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.