Biolegend, 154303, APC anti-mouse Perforin Antibody, 25μg

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse perforin transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone S16009A reacts with perforin from both C57BL/6 and BALB/c strains.
Product Citations: Cai Z, et al. 2023. Adv Sci (Weinh). 10:e2207155. PubMed Wang F, et al. 2023. Int J Mol Sci. 24:. PubMed Jiang X, et al. 2023. J Clin Invest. 133:. PubMed Read KA, et al. 2023. Nat Commun. 14:1652. PubMed Corria-Osorio J, et al. 2023. Nat Immunol. 24:869. PubMed Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed Alsaleh G, et al. 2020. Elife. 9: . PubMed Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed Zhang X, et al. 2021. Mol Cancer Res. 19:1076. PubMed Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed Haque A, et al. 2020. Exp Neurol. 330:113315. PubMed Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed Tao Z, et al. 2022. Cells. 11:. PubMed Wang F, et al. 2021. Neoplasia. 23:281. PubMed
RRID: AB_2721462 (BioLegend Cat. No. 154303) AB_2721463 (BioLegend Cat. No. 154304)
Structure: 70 kD Protein that oligomerizes for pore formation.
Distribution: CTL, NK (Cytoplasmic Granules)
Function: Target cell lysis
Cell Type: NK cells, T cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: Podack ER, et al. 1988. Immunol. Rev. 103:203. Konjar S, et al. 2010. Immunology. 131:257. Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735. Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.
Gene ID: 18646
UniProt: View information about Perforin on UniProt.org
Clone: S16009A
Regulatory Status: RUO
Other Names: Prf1, Pfp, Pfn, Pfn-1
Isotype: Rat IgG2a, κ