Biolegend, 154609, PE/Dazzle™ 594 anti-mouse GPR15/BOB Antibody, 25μg

GPR15, also known as BOB, is a G-protein coupled receptor with 7 transmembrane regions that is present on the cell surface as a homotrimer. Its ligand, GPR15L, was recently identified. GPR15 is expressed by effector T cells and subsets of memory T cells, and is involved in the recruitment of proinflammatory CD4 + T cells responsible of the pathogenesis in colitis. In humans, GPR15 acts as a receptor for human immunodeficiency virus (HIV) type 1 and 2, and is upregulated on monocytes and neutrophils in the synovia of patients with RA.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse GPR15-Transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2910318 (BioLegend Cat. No. 154609) AB_2910318 (BioLegend Cat. No. 154610)
Structure: G-protein coupled receptor
Distribution: Effector and memory T cells
Function: Probable chemokine receptor, signal transduction
Cell Type: T cells, Tregs
Biology Area: Immunology
Antigen References: Nguyen L, et al. 2015. Nature Immunology. 16:207 Kiene M, et al. 2014. PLoS. One. 9:e88195 Cartwright A, et al. 2014. Cytokine. 67:53 Kim SV, et al. 2013. Science. 340:1456 Okamoto Y, et al. 2013. J. Biol. Chem. 288:9189
Gene ID: 71223
UniProt: View information about GPR15/BOB on UniProt.org
Clone: S15042I
Regulatory Status: RUO
Other Names: BOB, Brother of Bonzo , G protein-coupled receptor 15, 4933439K08Rik
Isotype: Rat IgG2a, κ