Biolegend, 155506, PE anti-mouse CD170 (Siglec-F) Antibody, 100μg

CD170, also known as Siglec-F and Siglec-5, is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family of type I single pass transmembrane proteins.  Siglec-F has four extracellular Ig-like domains and two cytoplasmic ITIM motifs and preferentially binds [alpha]-2,3-linked sialic acid. Siglec-F is expressed on eosinophils, alveolar macrophages and intestinal microfold (M) cells and induces apoptosis of lung eosinophils during allergic asthma.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Lin CH, et al. 2023. iScience. 26:106904. PubMed Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed Yang L, et al. 2023. Acta Pharmacol Sin. :. PubMed Ydens E, et al. 2020. Nat Neurosci. 23:676. PubMed Yilmaz B, et al. 2021. Cell Host Microbe. 29(4):650-663.e9. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Mathur S, et al. 2021. JCI Insight. 6:. PubMed Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed Baasch S, et al. 2021. Cell. . PubMed Guo P, et al. 2021. J Immunol. 207:408. PubMed Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed Roberts LM, et al. 2021. iScience. 24:103025. PubMed Machácek T, et al. 2022. PLoS Pathog. 18:e1010302. PubMed Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed Penkert RR, et al. 2020. Obesity (Silver Spring). 1631:28. PubMed Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed Li H, et al. 2021. J Clin Invest. 131:. PubMed
RRID: AB_2750234 (BioLegend Cat. No. 155505) AB_2750235 (BioLegend Cat. No. 155506)
Structure: Member of the Sialic acid-binding Ig-like lectin family, type I, single pass, transmembrane protein, 4 extracellular Ig-like domains, 2 ITIM motifs in the cytoplasmic domain
Distribution: Eosinophils, alveolar macrophages, intestinal microfold (M) cells
Function: Induces apoptosis in eosinophils
Ligand/Receptor: [alpha]-2,3-linked sialic acid
Cell Type: Eosinophils, Macrophages
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: Gicheva N, et al. 2016. Biochem. Biophys. Res. Commun. 479:1. Kiwamoto T, et al. 2015. J. Allergy Clin. Immunol. 135:1329. Suzukawa M, et al. 2013. J. Immunol. 190:5939. Patnode ML, et al. 2013. J. Biol. Chem. 288:26533.
Gene ID: 233186
UniProt: View information about CD170 on UniProt.org
Clone: S17007L
Regulatory Status: RUO
Other Names: Siglec-5
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.