Biolegend, 156303, PE anti-mouse TCR vβ8.3 Antibody, 25μg

The TCR Vβ8 family consists of three members, namely Vβ8.1, 8.2 and 8.3. 1B3.3 reacts with Vβ8.3 only, but not Vβ8.1 or Vβ8.2. Most mouse strains have about 20% Vβ8+ T cells in the periphery. However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrc haplotype) do not express Vβ8 due to a gene deletion at the Vβ8 loci.  Exogenous superantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vβ8 and selectively eliminate those T cells in vivo .
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: 3.L2 T Cell Clone Expressing the vβ8.3 TCR
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone 1B3.3 recognizes TCR vß8.3 from both C57BL/6 and BALB/c strains.
Application References(PubMed link indicates BioLegend citation): Kersh G, et al. 1998. J. Immunol. 161:585.
Product Citations: Fu X, et al. 2020. J Immunol. 205:2066. PubMed Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
RRID: AB_2800699 (BioLegend Cat. No. 156303) AB_2800700 (BioLegend Cat. No. 156304)
Structure: Ig super family; TCR is an 85-kd hetero-dimer composed of alpha and beta chain.
Distribution: Expressed in a subset of αβ T cells
Function: Recognition of Peptide/MHC, T cell activation
Interaction: CD3
Ligand/Receptor: Peptide/MHC complex
Cell Targets: CD3
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: Haskins K, et al. 1984. J. Exp. Med. 160:452 Davis MM, et al. 1998. Annu Rev Immunol. 16:523 Kersh GJ, et al. 1998. J Immunol. 161:585. Tomonari K. 1996. Immunogenetics. 44:73
Gene ID: 21577
UniProt: View information about TCR Vbeta8.3 on UniProt.org
Clone: 1B3.3
Regulatory Status: RUO
Other Names: Tib, TCRB, TCRbeta
Isotype: Armenian Hamster IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.