Biolegend, 156403, PE anti-mouse CD32 (Fcgr2) Antibody, 25μg

CD32 (Fcgr2) is a 40 kD transmembrane glycoprotein, member of the immunoglobulin superfamily. The extracellular region of CD32 consists of two Ig C-type domains that binds the Fc region from monomeric IgG with low affinity, but binds immune complexes efficiently. CD32 can mediate phagocytosis of immune complexes and modulate cell activation. CD32 is expressed by Macrophages, neutrophils, mast cells and B cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD32 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Wang YJ, et al. 2023. Nat Commun. 7903:14. PubMed Chang S, et al. 2022. Phytother Res. 36:1678. PubMed
RRID: AB_2876522 (BioLegend Cat. No. 156403) AB_2876522 (BioLegend Cat. No. 156404)
Structure: 40 kD transmembrane glycoprotein, member of the immunoglobulin superfamily; extracellular region consists of two Ig C-type domains.
Distribution: Macrophages, neutrophils, mast cells and B cells.
Function: Mediates phagocytosis of immune complexes, modulates cell activation.
Ligand/Receptor: Binds Fc from monomeric IgG with low affinity, binds immune complexes efficiently.
Cell Type: B cells, Macrophages, Mast cells, Neutrophils
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Negishi-Koga T, et al. 2015. Nat Commun. 6:6637 Yamada DH, et al. 2015. Immunity. 42:379 Clatworthy MR, et al. 2014. Nat Med. 20:1458 Li F and Ravetch JV. 2011. Science. 333:1030 Xiang Z, et al. 2007. Nat Immunol. 8:419
Gene ID: 14130
UniProt: View information about CD32 on UniProt.org
Clone: S17012B
Regulatory Status: RUO
Other Names: Fcr-2, Fcr-3, Ly-17, LyM-1, Lym-1, fcRII, FcgRII, fcgr2b
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.