Biolegend, 156535, PE/Fire™ 810 anti-mouse NK-1.1 Antibody, 25μg

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo . NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse NK1.1-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm. Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S17016D cross-blocks anti-mouse NK1.1 clone PK136, and can stain for NK1.1 post-formaldehyde and methanol-based fixation based on in-house testing.
RRID: AB_3068267 (BioLegend Cat. No. 156535)
Structure: NKR-P1 gene family
Distribution: NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)
Function: NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Antigen References: Lanier LL. 1997. Immunity 6:371. Yokoyama WM, Seaman WE. 1993. Annu. Rev. Immunol. 11:613. Koo GC, et al. 1986. J. Immunol. 137:3742. Giorda R, Trucco M. 1991. J. Immunol. 147:1701.
Gene ID: 17059
UniProt: View information about NK-1.1 on UniProt.org
Clone: S17016D
Regulatory Status: RUO
Other Names: NKR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
Isotype: Mouse IgG2a, κ